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Reprinted from FEDERATION PROCEEDINGS Vol. 19, No, 1, Pt. I March 1960
Printed in United States of America
EFFECTS OF OUAFAIK OF K AND NA CONCENTRATIONS IN RESTING RAPEIT AURICLES. P. N. Kitt and R. S. Tuttle». Dept, of Pharmacology, State Univ. of N.Y., Upstate Medical Center, Syracuse, N. Y.
A relationship vas sought between dose of cardiac glycoside and its K depleting effect. In control experiments left rabbit auricles incubated for 2 hours at 37°C in Tyrode*s solution gradually lost K (31%) and gained Na (202%) as determined in the digests by flame photometry# Ouabain 10″‘ produced, in beating auricles, a steady increase in contractility. In resting auricles it diminished the 2 hour K loss to 13% while Na concentration was not above controls. 10″* ouabain which produced failure in beating auricles in 30 minutes decreased intracellular K concentration in 2 hours 54% and increased intracellular Na 202%. These results can be interpreted as meaning that a low or “therapeutic” glycoside concentration prevents K loss which is observed under control experimental conditions. On the other hand concentrations of ouabain which are toxic deplete the auricles of K and allow Na éntry into the cells. These effects of different concentrations of ouabain may explain discrepancies in the literature. (Supported by a grant from the American Heart Association).